No signal on Western blot, what to do next?
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Western blot, one of most commonly used laboratory techniques for protein work, is quite complex and many factors can influence its outcome. In case of problems with the detection of a target protein, the following can be done: |
 Left: before optimization of western blot conditions Right: after optimization of western blot conditions For catalog antibodies, we recommend checking the product information sheet for suggested experimental conditions, including sample type, protein load and more. You are always welcome to contact Agrisera Technical Support and we will be happy to help. |